Anti-PINK1 Antibody (56548) from QED Bioscience Inc.

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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (56548). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (56548) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) PINK1 Antibody (D) Composite.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (56548). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (56548) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) PINK1 Antibody. (D) Composite.
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Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (56548). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (56548) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) PINK1 Antibody. (D) Composite.
Western Blot

Western Blot analysis of Rat Brain showing detection of ~50 kDa PINK1 protein using Mouse Anti-PINK1 Monoclonal Antibody, Clone S4-15 (56548). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-PINK1 Monoclonal Antibody (56548) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~50 kDa.

Supplier Page from
QED Bioscience Inc. for
Anti-PINK1 Antibody (56548)

The Anti-PINK1 Antibody (56548) from QED Bioscience Inc. is a Mouse Monoclonal antibody to PINK1, and PTEN induced kinase 1. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Anti-PINK1 Antibody (56548) has been validated for the following applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blot.

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Western Blot

Anti-PINK1 Antibody (56548) from QED Bioscience Inc.

QED Bioscience Inc.

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Anti-PINK1 Antibody (56548) from QED Bioscience Inc.

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